Antagonistic activity of Bacillus velezensis SDTB038 against Phytophthora infestans in potato.
Identifieur interne : 000301 ( Main/Exploration ); précédent : 000300; suivant : 000302Antagonistic activity of Bacillus velezensis SDTB038 against Phytophthora infestans in potato.
Auteurs : Haohao Yan [Oman] ; Yue Qiu [Oman] ; Shuai Yang [Oman] ; Yongqiang Wang [Oman] ; Kaiyun Wang [République populaire de Chine] ; Lili Jiang [Oman] ; Hongyan Wang [Oman]Source :
- Plant disease [ 0191-2917 ] ; 2020.
Abstract
Potato late blight is a severe and highly epidemic disease caused by Phytophthora infestans that can affect all parts of the plant. This study mainly screened antagonistic strains for good control of potato late blight and identified strain SDTB038 as Bacillus velezensis according to its morphological and chemical properties and the 16S rRNA, gyrA and gyrB gene sequences. This antagonistic strain achieved good control of potato late blight in greenhouses and fields and promoted potato plant growth. Two-year field trials (2018, 2019) showed that B. velezensis SDTB038 can be used to reduce food losses caused by late blight, achieving late blight reductions of 40.79% (2018) and 37.67% (2019). In two-year field trials, the control effects of the highest concentrations of fluopimomide and B. velezensis SDTB038 were better than those of the other treatments. The control effect of 85 g ha-1 fluopimomide and B. velezensis SDTB038 and that of 170 g ha-1 fluopimomide alone showed no significant differences. These field results indicate that a low concentration of fungicide and a high concentration of SDTB038 can be effective in controlling potato late blight. Foliar detection showed that lipopeptides (LPs) have an inhibitory effect on P. infestans. The amplification of LP genes revealed surfactin (srfAB, srfAC) and fengycin (fenB) genes in SDTB038, but only surfactin production by B. velezensis SDTB038 was observed by UPLC-Q-TOF-MS analysis. Therefore, the strain B. velezensis SDTB038 can produce secondary metabolites that help potato plants resist late blight development, can effectively inhibit the infection of potato leaves by P. infestans, and has potential value for development as a biological pesticide against potato late blight.
DOI: 10.1094/PDIS-08-20-1666-RE
PubMed: 33174798
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17863801132@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Tai'an'</wicri:regionArea></affiliation></author><author><name sortKey="Qiu, Yue" sort="Qiu, Yue" uniqKey="Qiu Y" first="Yue" last="Qiu">Yue Qiu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong, China; qy18864805897@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Shuai" sort="Yang, Shuai" uniqKey="Yang S" first="Shuai" last="Yang">Shuai Yang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; sdauyys@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Shandong Agricultural University, 34734, Tai'an, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yongqiang" sort="Wang, Yongqiang" uniqKey="Wang Y" first="Yongqiang" last="Wang">Yongqiang Wang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; yqking997@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Shandong Agricultural University, 34734, Tai'an, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Kaiyun" sort="Wang, Kaiyun" uniqKey="Wang K" first="Kaiyun" last="Wang">Kaiyun Wang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; kywang@sdau.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>Shandong Agricultural University, 34734, Tai'an, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Lili" sort="Jiang, Lili" uniqKey="Jiang L" first="Lili" last="Jiang">Lili Jiang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agronomy, Shandong Agricultural University, 61 Daizong StreetTai'an, Shandong, China, 271018; j--lili@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Department of Agronomy, Shandong Agricultural University, 61 Daizong StreetTai'an, Shandong, China</wicri:regionArea><wicri:noRegion>China</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Hongyan" sort="Wang, Hongyan" uniqKey="Wang H" first="Hongyan" last="Wang">Hongyan Wang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong, China; sdauwhy@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato late blight is a severe and highly epidemic disease caused by Phytophthora infestans that can affect all parts of the plant. This study mainly screened antagonistic strains for good control of potato late blight and identified strain SDTB038 as Bacillus velezensis according to its morphological and chemical properties and the 16S rRNA, gyrA and gyrB gene sequences. This antagonistic strain achieved good control of potato late blight in greenhouses and fields and promoted potato plant growth. Two-year field trials (2018, 2019) showed that B. velezensis SDTB038 can be used to reduce food losses caused by late blight, achieving late blight reductions of 40.79% (2018) and 37.67% (2019). In two-year field trials, the control effects of the highest concentrations of fluopimomide and B. velezensis SDTB038 were better than those of the other treatments. The control effect of 85 g ha-1 fluopimomide and B. velezensis SDTB038 and that of 170 g ha-1 fluopimomide alone showed no significant differences. These field results indicate that a low concentration of fungicide and a high concentration of SDTB038 can be effective in controlling potato late blight. Foliar detection showed that lipopeptides (LPs) have an inhibitory effect on P. infestans. The amplification of LP genes revealed surfactin (srfAB, srfAC) and fengycin (fenB) genes in SDTB038, but only surfactin production by B. velezensis SDTB038 was observed by UPLC-Q-TOF-MS analysis. Therefore, the strain B. velezensis SDTB038 can produce secondary metabolites that help potato plants resist late blight development, can effectively inhibit the infection of potato leaves by P. infestans, and has potential value for development as a biological pesticide against potato late blight.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33174798</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Nov</Month><Day>11</Day></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Antagonistic activity of Bacillus velezensis SDTB038 against Phytophthora infestans in potato.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-08-20-1666-RE</ELocationID><Abstract><AbstractText>Potato late blight is a severe and highly epidemic disease caused by Phytophthora infestans that can affect all parts of the plant. This study mainly screened antagonistic strains for good control of potato late blight and identified strain SDTB038 as Bacillus velezensis according to its morphological and chemical properties and the 16S rRNA, gyrA and gyrB gene sequences. This antagonistic strain achieved good control of potato late blight in greenhouses and fields and promoted potato plant growth. Two-year field trials (2018, 2019) showed that B. velezensis SDTB038 can be used to reduce food losses caused by late blight, achieving late blight reductions of 40.79% (2018) and 37.67% (2019). In two-year field trials, the control effects of the highest concentrations of fluopimomide and B. velezensis SDTB038 were better than those of the other treatments. The control effect of 85 g ha-1 fluopimomide and B. velezensis SDTB038 and that of 170 g ha-1 fluopimomide alone showed no significant differences. These field results indicate that a low concentration of fungicide and a high concentration of SDTB038 can be effective in controlling potato late blight. Foliar detection showed that lipopeptides (LPs) have an inhibitory effect on P. infestans. The amplification of LP genes revealed surfactin (srfAB, srfAC) and fengycin (fenB) genes in SDTB038, but only surfactin production by B. velezensis SDTB038 was observed by UPLC-Q-TOF-MS analysis. Therefore, the strain B. velezensis SDTB038 can produce secondary metabolites that help potato plants resist late blight development, can effectively inhibit the infection of potato leaves by P. infestans, and has potential value for development as a biological pesticide against potato late blight.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Haohao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Tai'an', China; 17863801132@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong, China; qy18864805897@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Shuai</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; sdauyys@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yongqiang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; yqking997@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Kaiyun</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Shandong Agricultural University, 34734, Tai'an, Shandong, China; kywang@sdau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Lili</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Agronomy, Shandong Agricultural University, 61 Daizong StreetTai'an, Shandong, China, 271018; j--lili@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hongyan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Shandong Agricultural University, 34734, Plant Protection, Tai'an, Shandong, China; sdauwhy@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Causal Agent</Keyword><Keyword MajorTopicYN="N">Crop Type</Keyword><Keyword MajorTopicYN="N">Disease management</Keyword><Keyword MajorTopicYN="N">Field crops</Keyword><Keyword MajorTopicYN="N">Oomycetes</Keyword><Keyword MajorTopicYN="N">Subject Areas</Keyword><Keyword MajorTopicYN="N">cultural and biological practices</Keyword><Keyword MajorTopicYN="N">other</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>12</Hour><Minute>11</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33174798</ArticleId><ArticleId IdType="doi">10.1094/PDIS-08-20-1666-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Oman</li><li>République populaire de Chine</li></country></list><tree><country name="Oman"><noRegion><name sortKey="Yan, Haohao" sort="Yan, Haohao" uniqKey="Yan H" first="Haohao" last="Yan">Haohao Yan</name></noRegion><name sortKey="Jiang, Lili" sort="Jiang, Lili" uniqKey="Jiang L" first="Lili" last="Jiang">Lili Jiang</name><name sortKey="Qiu, Yue" sort="Qiu, Yue" uniqKey="Qiu Y" first="Yue" last="Qiu">Yue Qiu</name><name sortKey="Wang, Hongyan" sort="Wang, Hongyan" uniqKey="Wang H" first="Hongyan" last="Wang">Hongyan Wang</name><name sortKey="Wang, Yongqiang" sort="Wang, Yongqiang" uniqKey="Wang Y" first="Yongqiang" last="Wang">Yongqiang Wang</name><name sortKey="Yang, Shuai" sort="Yang, Shuai" uniqKey="Yang S" first="Shuai" last="Yang">Shuai Yang</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Kaiyun" sort="Wang, Kaiyun" uniqKey="Wang K" first="Kaiyun" last="Wang">Kaiyun Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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